BIO PROCESS ENGINEERING Question Papers (Regular, 2006)

SET: 1

1. Mention about the regulatory constraints of bioprocess.

2. a) Briefly explain about the materials of construction for fermentor fabrication.

b) Explain different types of agitators used in fermentors.

3. a) What are the major sources of carbon, nitrogen and phosphorous in industrial fermentation.

b) What are chelating agents? Explain their functions giving example.

4. a) Explain reactions involved that lead to loss of nutrient quality during sterilization.

b) Describe methods of batch sterilization.

5. Discuss the following:

(a) Medium formulation

(b) Yield factor.

6. Explain the synthesis of glucose from pyruvate(repeat , 2006, supplie, set:2 –Q6.)

7. Give short note on:

(a) Lag phase

(b) Logarithmic phase

(c) Stationary phase

(d) Death phase

8. Explain the difference between:

(a) Competitive and non competitive product inhibition

(b) Growth and non growth associated products

SET: 2

1. Mention different types of enzymes extracted from plant and their application

2. a) Briefly explain about the material of construction for fermentor fabrication

b) Explain different types of agitators used in fermentors

3. Explain the factors influencing the choice of carbon source.

4. Derive the mathematical expression for Nutrient Quality Criterion (Q) in continuous sterilization and describe the graphical method to optimize temperature-time regime.

5. Discuss the following n detail :

(a) Stoichiometrically limiting compounds.

(b) Growth rate limiting medium

6. Briefly discuss the following:

(a) Energy capture efficiency

(b) Oxygen consumption and heat evolution in aerobic cultures

(c) Heat generation and yield factor estimation

7. Give note on the following:

(a) Substrate limiting growth

(b) Unbalanced growth

(c) Balanced growth

8. Describe growth associated product formation with equation.

SET: 4

1. Write a short notes on:

(a) Transformation process

(b) Microbial metabolites

2. In a fed batch culture operating with an intermittent addition of glucose solution , values of the following parameters are given at time t = 2hrs, when the system is at quasi steady state. Culture volume(V) = 1 Litre, Substrate concentration(S0) = 100 gm glucose/litre, Limiting rate constant(Ks) = 0.1 gm glucose/lit, Initial amount of biomass in the reactor (X0t) = 30 gm, Nutrient feed flow rate (F) = dv/dt = 200 ml/h, Maximum specific growth rate = 0.3h-1 , yield coefficient(YM X/M) = 0.5 GM/ GM GLUCOSE

Determine :

(a) The initial culture of the medium (VO)

(b) Determine the concentration of the growth limiting substrate in the vessel at quasi steady state.

(c) Determine the concentration and total amount of biomass in the vessel at t = 2hrs(at quasi steady state).

(d) If specific rate of product formation (qp) = 0.2 gm product/ cells, PO = 0, determine the concentration of the product in the vessel at t = 2 hrs.

3. Explain the use of water as an important constituent for fermentation.

Describe the use of buffers for media preparation in fermentation.

4. Describe Arrhenius plot for the calculation of activation energy and derive an expression for heat sterilization of a pure culture at a constant temperature.

5. Determine the degree of reductions of substrate, bacteria, RQ, and yield coefficients for aerobic degradation of an organic compound by a mixed culture of organisms in waste water as represented by the following reaction

C3H6O3 + aO2 + bNH3 --------------- cC5H7NO2 + dH2O + eCO2

Determine the a, b, c, d and e, if YX/S = 0.4 g X/g S

6. Differentiate the major function of the dark and light phases in photosynthesis.

7. Enumerate the principle involved in the microbial growth taking an example.

Differentiate between the growth in the batch and continuous systems

8. Describe growth and non-growth associated products formation with equations.