Basics of micro array
1. ________ consists of an arrayed series of thousands of microscopic spots of DNA oligo nucleotides called features containing pico molecules of specific DNA sequences (S)
a. EST
b. CASP
c. CATH
d. DNA micro array ans-d
2. In DNA micro array features contains ________of specific DNA sequence (S)
a. milli molecules
b. micro molecules
c. pico molecules
d. nano molecules ans-c
3. In standard micro arrays, the probes are attached to a solid surface by a________ (S)
a. ionic bond
b. covalent bond
c. coordinate covalent bond
d. vander wall forces ans-b
4. __________chemical matrix is generally used in DNA micro arrays(S)
a. epoxy silane
b. aluminum silane
c. glutamic acid
d. silver nitrate ans-a
5. In_______ microscopic beads are used instead of large solid support(S)
a. colloquially affy chip
b. expony
c. gene chip
d. illumina ans-d
6. ________ is used to measure changes in expression levels (M)
a. DNA micro arrays
b. DNA macro arrays
c. SNP's
d. EST's ans-a
7. Micro array technology evolved from ____________ (M)
a. northern blotting
b. southern blotting
c. western blotting
d. eastern blotting ans-b
8. A complete eukaryotic genome (Saccharomyces cerevisiae) on micro array was published in (M)
a. 1950
b. 1960
c. 1997
d. 1897 ans-c
9. The use of collection of distinct DNA's in arrays for expression profiling was first described in (H)
a. 1987
b. 1887
c. 1667
d. 1567 ans-a
10. The process of measuring gene expression via cDNA is called (H)
a. Gene array
b. expression profiling
c. DNA chip
d. Genome chip ans-b
1. In micro array manufacture, array may have a range of ___________ (S)
a. 100 probes
b. 1 to 100000 probes
c. 10 to 2100000 probes
d. 500 to 100000000 probes ans-c
2. In oligo nucleotide micro arrays the probes are short sequences designed to match parts of the sequences of known or predicted_________(S)
a. restriction sites
b. virulence regions
c. RNA's
d. open reading frames ans-d
3. Agilent design has _________ probes in oligo nucleotide micro arrays (M)
a. 60-mer
b. 25-mer
c. 50-mer
d. 45-mer ans-a
4. ________mer probes are produced by affymetrix (H)
a. 60
b. 25
c. 50
d. 45 ans-b
5. A technique used to produce oligo nucleotide arrays include ______synthesis on a silica substrate, where light and light sensitive masking agents are used to 'build' a sequence one nucleotide at a time across the entire array (S)
a. photo lithographic
b. chemoo lithographic
c. organo lithographic
d. organo graphic ans-a
6. In two colour micro array ________ fluorescent dye is commonly used for cDNA labeling (M)
a. Cy3
b. Cy6
c. Cy8
d. Cy7 ans-a
7. Cy3 has a fluorescence emission wave length of _______nano meters (M)
a. 600
b. 550
c. 570
d. 450 ans-c
8. 570 nm corresponds to _______part of light spectrum (S)
a. red
b. green
c. violet
d. orange ans-b
9. Cy5 with 670nm corresponds to _______part of light spectrum (S)
a. red
b. green
c. violet
d. orange ans-a
10. Oligo nucleotide micro arrays often contain control probes designed to hybridized with (H)
a. DNA spike
b. RNA spike-ins
c. amino acid spike-ins
d. protein spike-ins ans-b
1. MAQC expand (S)
a. micro array quality control
b. macro array quality control
c. micro array quantity control
d. micro array quality construction ans-a
2. Data are more easily compared to arrays from different experiments in ________ micro array (S)
a. one colour
b. two colour
c. three colour
d. four colour ans-a
3. Gene expression values from micro array experiments can be represented as ______ to visualize the results of data analysis
a. cold map
b. heat map
c. lique map
d. kin map ans-b
4. _______ group is working on the standardization of the representation of gene expression data and relevant annotations (S)
a. MIAME
b. FDA
c. MAQC
d. MGED ans-d
5. ________ is also called as cell micro arrays (S)
a. tissue micro arrays
b. protein micro array
c. antibody micro array
d. transfection micro array ans-d
6. Which of the following eukaryotes complete genome on a micro array was published in 1997 (M)
a. fungus
b. Saccharomyces cerevisiae
c. pencillin
d. monkey ans-b
7. Expand MGED (M)
a. micro array and gene expression data
b. micro wave and gene expression data
c. micro array and genetic expression data
d. mini array and gene expression data ans-a
8. Statistical methods tailored to micro array analysis have recently become available that assess statistical power based on the variation present in the data and the number of experimental replicates and can help minimize _________ errors in analysis (H)
a. type I and Type II
b. type II and Type III
c. type IV
d. type V ans-a
9. Eppendrof 'dual chip and silver quant' is a popular ________ channel system (H)
a. double
b. single
c. triple
d. profiling ans-b
10. FDA stands for (M)
a. Food and drug administration
b. Food and drink administration
c. Forest development administration
d. Federal and drug administration ans-a
Primer on comparative genomics
1. Comparison of gene number, gene content and gene location in an organism refers to___________ (S)
a. genome
b. genomics
c. comparative genomics
d. comparison analysis ans- c
2. The genes which have function and evolutionary history are grouped under _______________ (S)
a. Orthologs
b. paralogs
c. homologs
d. heterologs ans- a
3. _______ comparision reveals number of gene families & duplicated genes (M)
a. one -against -all self
b. one-against-one self
c. all against
d. all-against-all-self ans- d
4. A value of por E___________ is usually the point at which the alignment score is no longer considered to be significant in order to force on a more closely related group of proteins (M)
a. > 0.01 to 0.05
b. <>
c. ≥ 0.01 to 0.05
d. ≤ 0.00 to 1 ans- a
5. In a method for clustering similar sequences that are likely to be paralogs ,the edges of E value which are __________ are removed. (H)
a. >1X10-6
b.>1X106
c. <1x106
d. <1x10-6 ans- a
6. Clustering by single linkage method is based on (S)
a. distance criterion
b. base criterion
c. amino acid manipulation criterion
d. base repetition criterion ans- a
7. ________ method represents core proteome of the organism from which all biological functions have diversified (M)
a. clustering by single linkage
b. clustering by making sub graphs
c. core proteome
d. clustering analysis ans- c
8. Two proteins ,X in proteome A & Y in proteome B are predicted to be an orthologous when E value for alignment should be ________ & the alignment should extended over ________ of each protein (H)
a. <0.01>
b. <0.01>
c. <0.01>
d. <0.01>
9. Best method for similarity search between yeast and worm is ________ (S)
a. WU-BLAST
b. j BLAST
c. z BLAST
d. p BLAST ans-a
10. Homology model are also used to generate hypothesis about the kinetics & dynamics of a protein ( give an example) (H)
a. Na+/K+ ATPase
b. potassium channel
c. osmoregulation
d. diffusion ans-b
Understanding one, detailed process of evolution at gross and local level & the other, translate DNA sequence data into proteins of known function are the two drivers of______ (S)
a. comparative genomics
b. genetics
c. molecular biology
d. computational biology ans- a
2. By mid of 2004 comparative genomics of bacteria listed______ number of bacterial genome sequences (S)
a. 100
b. 200
c. 173
d. 450 ans- c
3. Simple sequence data analysis revealed that the gene density is generally ________(S)
a. One gene per kilo base of DNA
b. One gene per 10 kilo base of DNA
c. 10 genes per kilo base of DNA
d. One gene per kilo base of protein ans-a
4. The minimal gene set consistent with the independent existence can be determined using________ (S)
a. comparative genomics
b. genetics
c. molecular biology
d. computational biology ans- a
5. Expand ORF (S)
a. open restriction fragments
b. ordered restriction fragments
c. open reading frames
d. ordered restriction frames ans- c
6. Comparative analysis of related bacteria showed that the genomic backbone is ______
a. heterologous (M)
b. paralogous
c. orthologous
d. homologous ans- d
7. Comparative analysis revealed that the chromosomal inversions in closely related bacteria are most likely to occur around the (M)
a. origin of replication
b. origin or terminus of replication
c. terminus of replication
d. translation ans- b
8. _________ bacterium is characterized by it's ability to survive extremely high doses of ionizing radiation (H)
a. Dienococcus radiodurans
b. S. cervecie
c. E.coli
d. algae ans-a
9. One of the following organelles DNA is large when compared to others (M)
a. fungi
b. animals
c. bacteria
d. plants ans- d
10. An example of eukaryote to study comparative genomics (H)
a. Encephalitozon cuniculi
b. E.coli
c. plants
0 comments:
Post a Comment