INSTRUMENTAL METHODS OF ANALYSIS Question Papers (Regular, 2007)

SET : 1

1. Give an account of the instruments involved in the electrochemical methods of analysis along wuth their principle of working.

2. Narrate the principle of operation of a fluorescence microscope. Give its applications in biology?

3. A protein has a sedimentation coefficient value of 3.12 × 10^-13 sec in water. Its diffusion coefficient in water is found to be 8.2 × 10^-7 /cm. both the above values have been corrected for 20C in water. The partial specific volume of the protein is 0.735, & the density of water at 20C is 0.9982. Determine the molecular weight of the protein?

Explain the principle involved in the above method.

4. Discuss the effect of hydrogen bonding in UV absorption. Explain by taking example.

What is the effect of electronegativity in UV absorption. Explain by taking examples of methyl chloride & methyl iodide.

5. What are fluorometric reagents? Mention some important fluorometric reagents.

How is fluorometric is used in the determination of thiamine in a meat sample?

6. Write short notes on :

a) Brainbridge mass spectrograph

b) Dempsters mass spectrometer

7. Write a brief account on :

a) Pulse – acquired method

b) Fourier transform method

c) Multidimensional NMR

8. Why electron spin resonance is also called electron paramagnetic resonance?

Calculate the ESR frequency of an unpaired electron in a magnetic field 0.33τ. Given, for electron g = 2 and β = 9.273 × 10^-24 J/T