METABOLIC ENGINEERING (Elective – II) syllabus, JNTU (2007-2008)

UNIT I : INTRODUCTION

Identification of metabolic regulation is a key point in metabolic engineering.

Basic concepts of Metabolic Engineering – Overview of cellular metabolism – Different models for cellular reactions, induction – Jacob Monod model and its regulation, Differential regulation by isoenzymes, Feed back regulation.

UNIT II : SYNTHESIS OF PRIMARY METABOLITES

Amino acid synthesis pathways and its regulation at enzyme level and whole cell level, Alteration of feed back regulation, Limiting accumulation of endproducts.

UNIT III : BIOSYNTHESIS OF SECONDARY METABOLITES

Regulation of secondary metabolite pathways, precursor effects, prophase, idiophase relationship, Catabolite regulation by passing control of secondary metabolism, producers of secondary metabolites, applications of secondary metabolites.

UNIT IV : BIOCONVERSIONS

Applications of Bioconversions, Factors affecting bioconversions, Specificity, Yields, Co metabolism, Product inhibition, mixed or sequential bioconversions, Conversion of insoluble substances.

UNIT V : REGULATION OF ENZYME PRODUCTIOn

Strain selection, Genetic improvement of strains, Gene dosage, metabolic pathway manipulations to improve fermentation, Feed back repression, Catabolite Repression, optimization and control of metabolic activities. The modification of existing - or the introduction of entirely new - metabolic pathways

UNIT VI : METABOLIC FLUX

Integration of anabolism and catabolism, metabolic flux distribution analysisbioprocess, material balance, kinetic types, equilibrium reaction. Experimental determination method of flux distribution, Metabolic flux analysis and its applications, Thermodynamics of cellular processes

UNIT VII : METABOLIC ENGINEERING WITH BIOINFORMATICS

Metabolic pathway modeling, Analysis of metabolic control and the structure

metabolic networks, Metabolic pathway synthesis algorithms,

UNIT VLLL : APPLICATIONS OF METABOLIC ENGINERRING

Application in pharmaceuticals, chemical bioprocess, food technology, agriculture,

environmental bioremediation and biomass conversion.

TEXT BOOKS:

1. Wang.D.I.C Cooney C.L., Demain A.L., Dunnil.P. Humphrey A.E. Lilly M.D.,

Fermentation and Enzyme Technology, John Wiley and sons 1980.

2. Stanbury P.F., and Whitaker A., Principles of Fermentation Technology,

Pergamon Press, 1984.

References :

Zubay G., Biochemistry, Macmillan Publishers, 1989.

http://ocw.osaka-u.ac.jp/contents/19/ME040512.pdf For unit Vl & Vll

http://ocw.osaka-u.ac.jp/contents/19/ME040421.pdf For unit Vl

http://ocw.osaka-u.ac.jp/contents/19/ME040526.pdf For unit Vll

http://ocw.osaka-u.ac.jp/contents/19/ME040602.pdf For unit Vl & Vll

http://www.bioinfo.de/isb/gcb01/poster/hurlebaus.html

How will bioinformatics influence metabolic engineering? Biotechnol Bioeng. 1998

Apr 20-May 5;58(2-3):162-9. For unit Vll