MICROBIOLOGY Question Papers (SUPPLE, 2008)

SET : 1

1. Explain the importance of solid culture media in development of microbiology.

2. What are “signature sequences” and polymerase chain reaction? How are they useful in classification of bacteria?

3. Describe the major classes in fungi with examples.

Explain the differences in major classes in fungi wit examples.

4. How is energy supplied to drive a concentration gradient across the membrane of the cell?

5. Describe the methodology for the enrichment of the following bacteria :

a) Thoibacillus thiooxidans

b) Rhizobium

c) Halobacterium

d) Clostridium

6. Define water activity. Explain how water influences the growth of different microorganisms?

7. Describe the different methods of helozetion?

What is the principle involved in an autoclave?

8. What are the tests performed to test drinking water quality?

What are the common contaminants associated with potable water?

Mention one method to prepare safe drinking water.

SET : 2

1. Describe the history and the development of virology.

2. What are “signature sequences” and polymerase chain reaction? How are they useful in classification of bacteria? (Repeat)

3. Describe the general characteristics of ricketsia.

4. How is the energy supplied to drive concentration gradient across the membrane of the cell? (R)

5. What is the generation time of a given microbe culture? Derive an equation for calculating the generation time when a bacterial organism divides by binary fission in exponential growth.

6. What is water activity? Explain the importance of water activity on microbial life and its preservation.

7. How are disinfectants evaluated?

Explain the mechanism of action of antimicrobial agents with suitable examples?

8. What is a standard operating procedure? Develop an SOP for an autoclaving.

SET : 3

1. Describe the history and development of virology. (R)

2. Define a “bacterial species”. Explain the difficulties encountered in classification of bacteria.

3. Describe the general characteristics of Chlamydia.

4. How is the energy supplied to drive concentration gradient across the membrane of the cell? (R)

5. What is pure culture of microorganisms? Explain different methods used in development of pure cultures.

6. Define water activity. Explain how water influences the growth of different microorganisms? (R)

7. Distinguish the terms, disinfectant and sterilization and explain the terms, disinfectant, sterilant and antiseptic with suitable examples.

8. How do you minimize the microbial population in a production block in an industry?

SET : 4

1. Describe the history and development of virology. (R)

2. Define a “bacterial species”. Explain the difficulties encountered in classification of bacteria. (R)

3. Write a short notes on the following :

a) Dimorphism in mucor.

b) Asexual reproduction of schizo saccaromyces.

c) Budding in saecharomyces.

d) Yeast hybridization.

4. How is the energy supplied to drive concentration gradient across the membrane of the cell? (R)

5. Describe the methodology for the enrichment of the following bacteria : (R)

e) Thoibacillus thiooxidans

f) Rhizobium

g) Halobacterium

h) Clostridium

6. Distinguish between a primary culture and stock culture.

Explain how sporulating organisms are preserved using soil.

Discuss about the use of a nutrient agar slant and nutrient agar stab in a bacteriology laboratory.

7. List out the most suitable disinfectant or antiseptic used for treating the following materials and justify the selection of a particular chemical

a) A patch of skin before injection

b) Drinking water

c) Laboratory bench top

d) Forceps

8. Mention standard quality control parameters for pharmaceuticals products?

How are they evaluated?