MOLECULAR BIOLOGY Question Papers (Supple,2008)

SET : 1

1. Describe the roles of different proteins in DNA replication.

2. DNA replication is bi-directional. What would the replication bubble look like

(using the same labeling regimen as discussed in class) if replication was unidirectional?

3. How does the structure of a eukaryotic CoreRNAPol compare with the E. coli CoreRNApol?

4. Why would isoleucine be less likely to occur in a beta turn than in a beta strand?

5. What is meant by Reverse Genetics? How is useful?

6. Write a note on split genes.

7. Describe the conjugation process in F+ and F− bacteria.

8. What are Retroposons?

SET : 2

1. List and describe the function of the 10 subunits constituting DNA polymerase-III. Distinguish between the holoenzyme and the core enzyme.

2. What is the start site for DNA replication?

3. What are the differences between prokaryotic and eukaryotic mRNA(s)?

4. Describe the following terms: Transcription, translation, promoter.

5. Differentiate between point mutations that are transitions versus tranversions. Using the DNA bases (A, T, G and C) list the four types of transition and the eight types of transversions.

6. Write a note on split genes.

7. Distinguish among the three modes of recombination in bacteria.

8. What are the similarities between a Iysogenic bacterial cell and an animal tumor cell which has been transformed by an oncogenic (tumor-producing) virus?

SET : 3

1. In what component parts do DNA and RNA differs?

2. What is the start site for DNA replication?

3. In yeast tRNA splicing, what is the importance of secondary structure?

4. When a globular protein folds into its native conformation, there is an increase in order of the protein (i.e., a decrease in its entropy). Explain the types of energetic factors that overcome this unfavorable entropic effect to make protein folding possible.

5. Briefly describe physical, chemical and biological methods of developing new mutations.

6. Write a note on split genes.

7. Describe the conjugation process in F+ and F− bacteria.

8. How do transpons cause mutations?

SET : 4

1. What is the relationship between nucleosomes, 30 nm fibers and the scaffold structure with respect to the organization of DNA in the nucleus?

2. DNA replication is bi-directional. What would the replication bubble look like(using the same labeling regimen as discussed in class) if replication was unidirectional?

3. How does the structure of a eukaryotic CoreRNAPol compare with the E. coli CoreRNApol?

4. Which feature of the Genetic code is responsible for redundancy of Geretic Code.

5. What are mutagens? Classify the radiation, chemical mutagens that are affecting the organisms.

6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.

7. With respect to F+ and F− bacterial mating, answer the followings:

a) How was it established that physical contact was necessary?

b) How was it established that chromosome transfer was unidirectional?

8. Describe the Shapiro model of transposition. What are the roles of transposase, DNA polymeraseI, Ligase and resolvase?