PLANT BIOTECHNOLOGY syllabus, JNTU (2007-2008)

UNIT I TISSUE CULTURE

Introduction to cell and tissue culture; Tissue culture media (composition, preparation); Initiation and maintenance of callus and cell suspension culture, Somatic embryogenesis, organogenesis; Protoplast isolation culture and fusion;

UNIT II TISSUE CULTURE APPLICATIONS I

Production of haploids, Somaclonal variations, Germplasm conservation (Cryopreservation);

UNIT III TISSUE CULTURE APPLICATIONS II

Production of secondary metabolites from plant cell cultures; Processes for enhancing the production of secondary metabolites. Technology of plant cell culture for production of chemicals; Bioreactors systems and models for mass cultivation of plant cells;

UNIT IV PLANT TRANSFORMATION TECHNOLOGY

Agrobacterium mediated gene transfer; Agrobacterium based vectors, viral vectors and their application. Direct gene transfer methods; chemical methods, electroporation, microinjection, particle bombardment.

UNIT V PLANT GENETIC ENGINEERING FOR PRODUCTIVITY AND

PERFORMANCE I (BIOTIC STRESS)

Herbicide resistance, Insect resistance, Disease resistance, virus resistance,.

UNIT VI PLANT GENETIC ENGINEERING FOR PRODUCTIVITY AND

PERFORMANCE II (ABIOTIC STRESS)

Abiotic stress tolerance ; Drought, temperature, salt .

UNIT VII MOLECULAR FARMING & INDUSTRIAL PRODUCTS

Application of Plant biotechnology for the production of quality oil, Industrial enzymes, Therapeutic Proteins , Antigens (edible vaccine) and plantibodies.

UNIT VIII METABOLIC ENGINEERING

Metabolic engineering for plant primary metabolites and secondary metabolites.

Only sec metabolites for our batch.

TEXT BOOKS:

1. Roberta Smith, Plant Tissue Culture: Techniques and Experiments. 2nd ed., Academic Press, 2000.

2. Bhojwani, S.S. and Rajdan, Plant Tissue Culture: Theory and Practice. 2004

REFERENCES:

1. Crispeels, M.J. and Sadava, D.E., Plants, Genes and Crop Biotechnology,

Jones and Bartlett Publishers (2nd Edition), 2003.

2. Bhowjwani, S.S., Plant Tissue Culture: Application and Limitations. Amsterdam,

Elsevier,1990.

3. Charles Cunningham and Andrew J.R. Porter, Recombinant Proteins from Plants: Production and Isolation of Clinically

Useful Compounds (Methods in Biotechnology), Humana Press, 1997.

4. Bernard R. Glick and John E. Thompson, Methods in Plant Molecular Biology and Biotechnology, CRC Press, 1993.

5. I. Potrykus and G. Spangenberg, , Gene Transfer to Plants (Springer Lab Manual), Springer Verlag, 1997.

6. Peter M. Gresshoff, Plant Genome Analysis: Current Topics in Plant Molecular Biology. CRC Press, 1994.

7. John Hammond, Peter McGarvey, Vidadi Yusibov, Plant Biotechnology: New Products and Applications, Springer Verlag, 1999.