ENZYME ENGINEERING Question Papers (2007,Supple)

SET :1

1. State the importance of enzymes in medical diagnosis.
2. How enzymes are preliminarily purified from a crude enzyme extract?
3. Give an account of various factors that affect enzyme catalyzed reaction.
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed reaction.
5. Explain the kinetics of competitive inhibition. Add a note on its importance.
6. Write the merits and demerits of enzyme immobilization techniques.
7. Give an account of different types of immobilized enzyme reactors.
8. What are the different types of transducer used in biosensors?

SET :2

1. State the importance of coenzymes and cofactors in enzyme catalysis.
2. Describe the procedures of enzyme isolation from natural sources.
3. Distinguish between the lock & key and Induced Fit models for binding of a substrate to an enzyme.
4. Discuss about the Eadie-Hofstee and Hanes plot and state their edge over the LB plot.
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization. [16]
7. Enumerate the kinetics of immobilized enzymes.
8. Enumerate the application of enzymes in analysis.

SET :3

1. What are enzymes? Justify its role as biocatalysts.
2. Discuss the methods employed for isolating enzymes soluble in cytoplasm and subcellular organelles from animal, Plant and microbial sources.
3. Distinguish between the lock & key and Induced Fit models for binding of a substrate to an enzyme.
4. Discuss the kinetics for reversible reactions.
5. How Koshland-Nemethy-Filmer (KNF) model account for allosteric regulation.
6. Give an account of analytical application of immobilized enzymes.
7. Formulate and calculate dimensionless groups and effectiveness factor.
8. Enumerate the application of enzymes in analysis.

SET :4

1. Discuss enzyme classification in terms of four-digit classification number?
2. Describe the procedures of enzyme isolation from natural sources.
3. Explain the energetics of enzyme-substrate complex formation.
4. Derive Michaelis-Menten equation. State the importance of MM constant.
5. Explain various types of enzyme inhibition with relevant examples.
6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization.
7. Give an account of different types of immobilized enzyme reactors.
8. Enumerate the application of enzymes in analysis.