BIOSENSORS AN BIOELECTRONICS Question Papers (2008, Reg, R05)

SET :1

1. What are ion mediators? Explain the role of Ferrcenes in transporting the electrons to the transducers.
2. a) Write the advantages of microbial biosensors over bioaffinity biosensors. b) Explain the mechanism of analyte diffusion through membrane to reach the reaction site.
3. Write the principle of potentiometric transducers and explain its electron flow behaviour to measure the response.
4. Write in detail about the construction and operation of surface acoustic wave (SAW) transducers.
5. Explain in detail about the estimation of glucose by electrochemical biosensors in blood samples from diabetics.
6. Explain about the online monitoring process of the biological oxygen demand in fermentor using specifically designed biosensors.
7. Schematically explain how a reaction graph representing a molecular program execution in biomolecular computers.
8. It is possible to create a biological device, capable of learning and having multilevel architecture and a high degree of behavioral complexity, explain this assumption based on Belousov-Zhabotinsky media photosensitive processing.

SET :2

1. What is inactivation? Write in detail about various mechanisms of inactivation of biological materials.
2. Explain the following : a) Oxygen Electrode Transdcer b) Thick-Film screen-printing technique.
3. Illustrate the following with suitable examples. a) Refractive Index sensors. b) Optical wave guides.
4. GIve details about the fabrication and working of Surface Transverse Wave (STW) transducers.
5. Illustrate the method of estimating urea from blood sample by bioaffinity sensors.
6. Explain the process for continuous online monitoring of B.O.D using microbial sensors.
7. Explain the program of catalytic circuitry in biocomputing system.
8. Explain how we can develop local-search algorithms that are particularly suited to biocomputers.

SET :3

1. What are electron carriers? Explain the importance of electron carriers in biosensors and mention the advantages and disadvantages.
2. Explain how bioaffinity based biosensors are useful tools to identify microorganism based on species specific sequence DNA.
3. a) What are the limitations of Total internal reflectance flourescence sensors.
4. Define the following. a) Transducers made up of piezo electric ceramics b) Role of magnetostrrictive material in piezo electric transducers.
5. Explain the assay of cholesterol in blood and body tissue using electrochemical affinity biosensors.
6. Detail about flow injection aperometric enzyme biosensor for direct determination of nuerotoxic agents.
7. Write the pattern of autocatalytic duplex formation by a cross-catalytic circuit.
8. Explain in detail about the logical design and logical operation of the Photonic biocomputers.

SET :4

1. What is interface and explain the interface chemistry involved between bioactive material and sensor material.
2. Explain various methods exist for manipulating the environment of native enzymes to improve the analytical stability in biosensors.
3. Name any five commercially available amperometric transducer sensors and explain their superiority over each other.
4. Explain the mechanism of operation of molecular electronic transducers.
5. Write about the method for serological diagnosis and estimation of nerve agents in bodyfluids by impedometric biosensors.
6. Explain the process for online monitoring of lactic acid in fermentation industry by biosensors.
7. What are molecular switches? Explain the importance of molecular switches in biomolecular electronics.
8. Discuss in detail parallel association memory (PAM) model in biomolecular computers.