BASIC INDUSTRIAL AND ENVIRONMENTAL BIOTECHNOLOGY syllabus, JNTU (2007-2008)

UnitI : Production of Primary Metabolites

A brief outline of processes for the production of some commercially important Organic acids (e.g. citric acid, lactic acid, acetic acid, gluconic acid,); Amino acids (Glutamic acid, lysine, aspartic acid&Phenylalanine); and Alcohols (ethanol, 2,3- butanediol)

UNIT II: Secondary Metabolites :

Study of production processes for various classes of low molecular weight secondary metabolites: Antibiotics-beta-lactams (Penicillins), semi synthetic Pencillins and Cephalosporins amino-glycosides (streptomycin), macrolids (erythromycin), quinines, and aromatics. Vitamin (B12) and Steroids, dual

or multiple fermentation.

Unit III: Production of Commercially Important Enzymes

Proteases, Amylases Lipases, Cellulases, Pectinases, Isomerases and other commercially important. Enzymes for the food pharmaceutical industries;

UNIT IV : Recombinant Proteins

Production of recombinant proteins (Insulin, Interleukin & Interferon’s ) having therapeutic and diagnostic applications; production of vaccines.

Unit V : Bio products and other Processes

Natural Biopreservatives (Nisin), and Biopolymers (Xanthan Gum and PHB); Single Cell Protein, Racemically-pure Drug Intermediates, Steroid Bioconversions; High -Fructose Corn syrup; Bioconversion of Vegetable Oils, Bioleaching.

Unit VI : Biological treatment of waste Water – Aerobic and Anaerobic Systems

Biological processes for domestic and industrial waste water treatments; Aerobic systems - activated sludge process, trickling filters, biological filters, rotating biological contractors (RBC), Fluidized bed reactor (FBR), expanded bed reactor, Inverse fluidized bed biofilm reactor (IFBBR) packed bed reactors air- sparged reactors ; Anaerobic Systems - contact digesters, packed column reactors, UASB.

Unit VII: Bioremediation

Introduction, constraints and priorities of Bioremediation, Biostimulation of Naturally occurring microbial activities, Bioaugmentation, in situ, ex situ, intrinsic & engineered bioremediation,Solid phase bioremediation - land farming, prepared beds, soil piles, Phytoremediation. Composting, Bioventing & Biosparging; Liquid phase bioremediation - suspended bioreactors, fixed biofilm reactors.

Unit VIII: Hazardous Waste Management

Introduction - Xenobiotic compounds, recalcitrance. hazardous wastes - biodegradation of Xenobiotics . Biological detoxification - hazardous waste management with at least 3 – 4 Examples.

TEXT BOOKS:

Biotechnology, 3rd edition by John E. Smith. Cambridge low price editions.

Industrial Microbiology: - J. E. Casida;

3 Environmental Biotechnology by S. K. Agarwal

4 Biodegradation & Bioremediation (1999), Martin Alexander, Academic press.

REFERENCES:

1.Microbiology: - Prescott and Dunn.

2.Microbial biotechnology: Glazer, A.N. and Nikaido, H. 1995 W.H. Freeman &Company, New York.

3. Industrial Microbiology:- A. H. Patel.. Stanier R. Y., Ingram J.L., Wheelis M.L., Painter R.R., General

Microbiology McMillan Publications, 1989.

4. Foster C.F., John Ware D.A., Environmental Biotechnology, Ellis Horwood Ltd.,1987.

5. Karrely D., Chakrabarty K., Omen G.S., Biotechnology and Biodegradation,

6. Advances in Applied Biotechnology Series, Vol.4, Gulf Publications Co. London, 1989.

7. Bioremediation engineering; design and application 1995 John. T. cookson,Jr. Mc Graw Hill, Inc.

8. Environmental Biotechnology by A.K. Chatterjee

9. Environmental Biotechnology by S.N.Jogdand Himalaya Publishing