BIOCHEMICAL REACTION ENGINEERING -I syllabus, JNTU (2007-2008)

UNIT I: Fundamentals of reaction engineerin :

Concept of order, molecularity of a reaction,searching a mechanism for a reaction, evaluation of rate constants, factors affecting reaction rates- pH, temperature using Arrhenius equation.

Unit II:Reactions involving cells-I

Growth Kinetics- batch, fed-batch and continuous mode of operation in reaction system, evaluation of kinetic parameters Monod’s equation- parameters, death rate of cell-batch and continuous sterilization.

UNIT III: Reactions involving cells-II

Influence of cell age distribution on growth kinetics, effect of inhibitors, substrate inhibition, Models for inhibition kinetics, Evaluation of parameters in Han-Levenspiel model.

Unit IV: Reactions involving cells-III

Stoichiometry of cell growth and product formation- elemental and available electron balances, degrees of reduction, maintainance coefficient, online data analysis for measurement of biochemical parameters, state and parameter estimation technique.

UNIT V: Multiple Reactions

Parallel series, series – parallel reactions, calculation of yield and selectivity, role of thermodynamic parameters, metabolic flux analysis, basic concepts of structured model and introduction to cybernetic models, Design principles- non isothermal reactions and pressure effects, concepts of residence time distribution, micromixing and macromixing

Unit VI: Mechanisms and Kinetics of Enzyme Action

Mechanisms of Enzyme Action; Concept of active site and energetics of enzyme Kinetics, substrate complex formation; Specificity of enzyme action; Kinetics of single substrate reactions; turnover number; estimation of Michaelis-Menten parameters. Importance of K_M, Multi-substrate reaction mechanisms and kinetics. Types of Inhibition- kinetic models; Substrate and Product Inhibition; Allosteric

regulation of enzymes; Deactivation kinetics.

Unit VII: Enzyme Immobilization

Physical and Chemical techniques for enzyme Immobilization - adsorption. matrix

entrapment, encapsulation. cross-linking. covalent binding - examples; Advantages

and disadvantages of different Immobilization techniques. overview of applications

of immobilized enzyme systems, effect of pH, temperature on immobilized reaction kinetics.

Unit VIII: Mass Transfer Effects In Immobilized Enzyme Systems and Design of

Enzyme Reactors

Analysis of Film and Pore Diffusion Effects on kinetics of Immobilized Enzyme

Reactions; Formulation of dimensionless groups and calculation of Effectiveness

Factors, Thiele modulus

Note: In all units relevant basic numerical problems should be practiced.

TEXT BOOKS:

Harvey W. Blanch, Douglas S. Clark Biochemical Engineering, Marcecel, Dekker, 2007.

O.Levenspiel. Chemical Reaction Engineering 3ed edition, wiley, Newyork, 1999.

P.M.Doran Bioprocess Engneering principles, academic Press, London, 1995.

D.G.Rao, Introduction to Biochemical Engineering, McGraw-Hill,2005

K.A. Gavhane, Chemical Reaction Engineering –I, Nirali Prakashan,2006

J. Nielsen, J. Villadsen and G. Liden, Bioreaction Engineering Principles, Springer International, 2^nd edition, 2003.

7. G.Hammes, Thermodynamics and kinetics for the biological sciences, Wiley – VCH, 2000

REFERENCES:

1. H. Scott Fogler, Elements of Chemical Reaction Engineering, II Edition, Prentice Hall of India Pvt. Ltd.

2.. J.M. Smith, Chemical Engineering Kinetics, Mc Graw Hill, 1981.

3. M.L. Shuler and F. Kargi Bioprocess Enggnerring: basic concepts.

4.. H.J. Fromm Initial rate Enzyme kinetics, Springer-verlog. Berlin 1975.