BASIC INDUSTRIAL BIOTECHNOLOGY Question Papers (Supple, 2004)

SET: 1

1. Enumerate unique features of biotechnology companies.

2. Briefly describe the over production methodologies used in fermentation indudustry?

3. Bring out the merits and demerits of surface and submerged culture with respect to citric acid production.

4. Write the importance of production medium in antibiotic production by microorganisms?

5. Describe the role of pectinase in food processing industries.

6. Define cellular yield coefficient ? Describe its importance in microbial growth and product formation.

7. Illustrate the role of Azolla as bio-fertilizer in rice crop improvement.

8. List out the different organisms that can be used as single cell proteins and give their optimum growth conditions.

SET: 2

1. Briefly describe the role of organisms in the production of novel biochemicals.

2. Describe different complex nitrogen sources and their importance in enhansing bio-product production.

3. List major industrially important organic acids produced by microbes and the producing organisms. Mention various raw materials used in industries for production of these compounds.

4. Explain the importance of side chain in the production of synthetic penicillin.

5. Describe the down stream processing for endocellular enzyme purification.

6. Write short notes on :

(a) Enthalpy

(b) Gibbs energy

(c) Biomass yield

7. How biotechnology can be beneficial for the conversion of vegetable oils to value added products.

8. Give the importance of methane, methanol, n-paraffins and agricultural wastes in single cell protein production.

SET: 3

1. Bring the role of a bioprocess engineer in the biotechnology industry.

2. Describe the necessity of inoculum development in fermentation process.

3. Write in detail the production phenylalanine by fermentation process and bring out its commercial importance.

4. Enumerate the function of penicillin as antibiotic compound.

5. Discuss the importance of quality control in production of pharmaceutically important enzymes.

6. Describe in detail:

a) Threshold concentration

b) Relation between biomass yield and product production

c) Estimation of growth stoichiometry

7. Enumerate the commercial importance of xanthan gum in various industrial sectors.

8. Explain downstream processing of penicillin with the help of a flow sheet.

SET: 4

1. List out some important organisms that can be used in industrial biotechnology.

2. What are the important criteria to select the agricultural waste material as raw material for fermentation?

3. Write the importance of agricultural raw materials in the production of various primary metabolites with regard to production economics.

4. Explain in detail the various steps involved in the production of penicillin on industrial scale.

5. Describe various types of proteases, producing organisms and their industrial application.

6. Discuss the following:

a) Microbial growth kinetics

b) Polymerase chain reaction

c) Parasexual cycle

7. Describe the outline details of xanthan gum biosynthetic process?

8. What are important racemically pure drug intermediates? How are they produced by biotechnological methods? Describe briefly the importance of microbes in production recemic compounds.