BASIC INDUSTRIAL BIOTECHNOLOGY Question Papers (Supple, 2006)

SET :1

1. Briefly write a historical overview of industrial fermentation process. [16]

2. Write short notes on:
(a) Sterilization.
(b) Contamination.
(c) Fermentation media screening. [6+5+5]

3. Give details of citric acid production pathway starting from glucose as substrate? [16]

4. Enumerate the role of precursors and inducers in secondary metabolite production? [16]

5. Name different industrially useful enzymes, source of organisms and growth conditions? [6+6+4]

6. (a) Discuss the principles of microbial growth.
(b) Describe the growth characteristics of a microorganism in a batch culture. [8+8]

7. Comment on:
(a) Commercially available BT formulations.
(b) Chemical insecticides versus biopesticides.
(c) Biopesticide role in crop protection. [6+5+5]

8. Write briefly note on:
(a) Chemical properties of interferons.
(b) Functional specificity of interferons.
(c) Uses of interferons. [6+5+5]

SET :2

1. Describe the role of following in modern industrial biotechnology:
(a) Production of chemicals.
(b) Pollution control.
(c) Human health care. [5+5+6]

2. Write down the importance of isolation of organisms for bioproduct production? [16]

3. Enumerate the importance of microbial species in organic acid production? [16]

4. (a) Bring out the role of vitamin B12 in the metabolism.
(b) Describe the method of production by fermentation.
(c) What are the microorganisms used for its production. [5+6+5]

5. (a) What are the steps involved in the production of enzymes.
(b) Illustrate with the help of a diagram. [8+8]

6. Write short notes on the following with relating to mutant development:
(a) NTG (nitrosoguanidine).
(b) UV irradiation.
(c) Ethylene di amine. [6+5+5]

7. Give detailed account of polyhydroxybutyrate production by microbial system
starting from glucose as carbon source? [16]

8. Describe briefly:
(a) Influenza vaccine.
(b) Hepatatis B vaccine.
(c) Polio vaccine.
(d) Diphtheria vaccine. [4x4]

SET :3

1. Enumerate the role of microorganisms in a bioprocess. [16]

2. What are the important criteria to select the agricultural waste materials as raw materials for fermentation? [16]

3. Write short notes on:
(a) Ratio of surface area to volume in citric acid production.
(b) Various steps involved in citric acid downstream process.
(c) Media composition for gluconic acid production. [4+6+6]

4. Discuss in detail the downstream processing methods used in the production of aromatic compounds? [16]

5. Describe the role of enzymes in food and pharmaceutical industry with examples? [16]

6. (a) Describe somatic hybridization with examples?
(b) Bring out the uses of protoplast fusion and somatic hybrids? [8+8]

7. Write an overview of importance of specially agricultural products in different sectors of industrial biotechnology? [16]

8. What is the importance of interferon in human health care and illustrate their production processes? [16]

SET :4

1. Write the importance of following in bioprocess development:
(a) Microbiological inputs.
(b) Bioreactor operation modes.
(c) Process parameters. [5+5+6]

2. Describe different complex nitrogen sources and their importance in enhancing bioproduct production? [8+8]

3. Write in detail the production of phenyl alanine by fermentation process and bring out its commercial importance? [10+6]

4. Discuss in detail the downstream processing methods used in the production of aromatic compounds? [16]

5. Discuss the importance of recombinant proteins in human health care sector? [16]

6. Describe the role of mutations in development of industrially important organisms and give examples? [12+4]

7. Write an overview of importance of specially agricultural products in different sectors of industrial biotechnology? [16]

8. Give a comparative account of vaccine production by traditional and modern methodologies? [16]