BASIC INDUSTRIAL BIOTECHNOLOGY Question Papers (Regular, 2006)

SET :1

1. Briefly write a historical overview of industrial fermentation process. [16]

2. Describe and discuss the characteristics of an ideal production medium? [4+12]

3. Write different processes involved in microbial conversion of ethanol to acetic acid? [16]

4. Discuss in detail the downstream processing methods used in the production of aromatic compounds? [16]

5. Enumerate the role of isomerases in production of pharmaceutically important compounds? [16]

6. (a) Discuss the principles of microbial growth.
(b) Describe the growth characteristics of a microorganism in a batch culture. [8+8]

7. Write short notes indicating their importance in biotechnology on:
(a) Gibberilla fugicora.
(b) Downstream process of hormone production.
(c) Vegetable oils. [6+5+5]

8. Write the importance of biological system in steroid transformations with respect to chemical transformations? [16]

SET :2

1. Describe the role of following in modern industrial biotechnology:
(a) Production of chemicals.
(b) Pollution control.
(c) Human health care. [5+5+6]

2. Describe the following:
(a) Working stock culture.
(b) Primary stock cultures.
(c) Lyophilized cultures. [6+5+5]

3. Describe the recovery process of gluconic acid from fermentation broth with the help of flow sheet? [16]

4. Enumerate the role of precursors and inducers in secondary metabolite production? [16]

5. Describe the downstream processing for endocellular enzyme purification? [16]

6. Write short notes on the following with relating to mutant development:
(a) NTG (nitrosoguanidine).
(b) UV irradiation.
(c) Ethylene di amine. [6+5+5]

7. Discuss the importance of plant growth hormones in transgenic plant development? [16]

8. Give the importance of recombinant DNA technology in viral vaccine development?

SET :3

1. Enumerate the importance of traditional technologies in modern biotechnology development. [16]

2. What are the important criteria to select the agricultural waste materials as raw materials for fermentation? [16]

3. Write short notes on production of :
(a) Lactic acid.
(b) Lysine.
(c) Ethanol. [6+5+5]

4. Enumerate the function of penicillin as antibiotic compounds? [16]

5. Write the importance of enzymes in economizing the industrial development? [16]

6. Describe the role of mutations in development of industrially important organisms and give examples? [12+4]

7. Give detailed account of polyhydroxybutyrate production by microbial system starting from glucose as carbon source? [16]

8. (a) What are semi synthetic penicillins.
(b) Give their importance over penicillins in disease control. [8+8]

SET :4

1. Describe the connective links for production of following:
(a) Glycolysis – acetic acid production
(b) TCA cycle – amino acid production
(c) Pyruvic acid – lactic acid production [6+5+5]

2. Describe the necessity of inoculum development in fermentation process? [16]

3. Briefly describe fermentation processes involved in the production of :
(a) Aspartic acid
(b) Gluconic acid
(c) Ethanol [5+5+6]

4. Discuss in detail the downstream processing methods used in the production of aromatic compounds? [16]

5. Describe the various steps involved in insulin production by recombinant microbial species? [16]

6. Describe the importance of the following with relation to industrial microbiology:
(a) Resistant mutants.
(b) Revertant mutants.
(c) Heterologous proteins. [5+5+6]

7. Write an overview of importance of specially agricultural products in different sectors of industrial biotechnology? [16]

8. Describe briefly:
(a) Influenza vaccine.
(b) Hepatatis B vaccine.
(c) Polio vaccine.
(d) Diphtheria vaccine. [4x4]