BIO PROCESS ENGINEERING Question Papers (Regular, 2004)

SET: 1

1. What is chromotagraphy? Explain the important chromatographic methods.

Draw the schematic diagram of a typical chromatography column and explain the working principle giving the general materialk balance.

2. What is bakers yeast? Explain briefly the process for producing bekers yeast.

What is high-fructose corn syrup? What are the commercial uses? Describe the process for producing high fructose corn syrup.

3. Explain the important points to be considered for medium formulation.

4. What is filter sterilization? Explain its application in media sterilization.

5. Explain the concept of degree of reduction and its application in proton- electron balance in bioreactor.

6. Explain the transfer of bioenergy via ATP.

7. Enumerate the various environmental factors that effect the growth kinetics.

8. Explain the substrate and product inhibition on the product formation with appropriate examples.

SET: 2

1. What are the important parameters to be considered for selecting a solvent in liquid – liquid extraction for inhibitory products?

Draw the flow diagram and write the material balance of a single stage extraction system and derive an expression for extraction factor.

2. What are the commercial uses of acetone and butanol? Name the species and substrates used for acetone – butanol production.

Draw the flow diagram for the process for acetone- butanol production. Name the other fermentation products, and micronutrients used in the process.

3. Give the composition of four industrially important media usedin industrial fermentation.

4. Describe the methods of sterilizing air in aerobic fermenors.

5. Explain in detail the stoichiometry involved in cell growth and product formation.

6. Enumerate the aerobic catabolism of glucose with emphasis on energetics.

7. Enumerate in detail various environmental conditions that effect the growth kinetics.

8. Briefly discuss about the structured models for product formation and compare with growth.

SET:3

1. Explain aqueous two phase extraction and its application in product recovery. Give examples.

Describe adsorption giving the schematic flow diagram and explain the industrial application.

2. Describe the process of ethanol production explaining the stoichiometry, names of organisms, sustrates and process conditions.

Describe the commercial uses of ethanol

3. Describe the use of precursors and metabolic regulators in media preperation.

4. Describing the methods for sterilizing fermentors

Explain the methodsof sterilizing variety of additives administered during the process of fermentation.

5. Discuss in detail the stoichiometry of product formation with an example.

6. What are the major steps in aerobic metabolism of hydrocarbons? What are the end products.

7. Explain specific growth rate with relevant equations.

8. Enumerate difference between growth and non growth associated product kinetics.

SET: 4

1. Describe a generalised flow sheet for fermentation process indicating the important upstream and downstream sections.

Describe the factors for the cost analysis of a industrial fermentation processes.

2. Describe the status and details of animal cell culture, the medium compositions, process conditions, contaminants, and major products involved.

What is meant by plant tissue culture? Explain the commercial importance of plant tissue culture giving examples of major products.

3. Describe in detail the theory of oxygen requirement and supply in industrial fermentation.

4. An autoclave malfunctions, and the temperature reaches only 119.5 C. the sterilization time at the maximum temperature was 20 minutes. The jar contains 10 litres of complex medium that has 105 spores/ litre. At 121 C specific death rate kd = 1.0 min-1 and activation energy for the death (Eod) = 90 kcal/g-mol. What is the probability that the medium was sterile?

5. Enumerate the elemental material balance of growth with an example.

6. Differentiate the heterotrophic and autotrophic metabolism with emphasis on energetics.

7. Discuss the kinetics of microbial growth and substrate consumption.

8. Critically evaluate the application of Luedejking-Piret model in bio process engineering.