BIOPROCESS ENGINEERING syllabus, JNTU (2007-2008)

UNIT I: INTRODUCTION TO BIOPROCESSES

An overview of traditional and modern applications of biotechnology industry, outline of an integrated bioprocess and the various (upstream and down stream) unit operations involved in bioprocesses, generalized process flow sheets.

UNIT II: FERMENTATION PROCESSES-I

General requirements of fermentation processes, Basic design and construction of fermentor and ancillaries, Main parameters to be monitored and controlled in fermentation processes;

UNIT III: FERMENTATION PROCESSES-II

An overview of aerobic and anaerobic fermentation processes and their application in the biotechnology industry, solid-substrate, slurry fermentation and its applications, whole cell immobilization, behaviour of microbes in different reactors (air lift, fluidized, batch, continuous fed batch condition).

UNIT IV: MEDIA DESIGN

Medium requirements for fermentation processes, carbon, nitrogen, minerals, vitamins and other complex nutrients, oxygen requirements, medium formulation for optimal growth and product formation, examples of simple and complex media, design and usage of various commercial media for industrial fermentations

UNIT V: : METABOLIC STOICHIOMETRY

Stoichiometry of Cell growth and product formation, elemental balances, degrees of reduction of substrate and biomass, available electron balances, yield coefficients of biomass and product formation, maintenance coefficients.

UNIT VI: ENERGETICS

Energetic analysis of microbial growth and product formation, oxygen consumption and heat evolution in aerobic cultures, thermodynamic efficiency of growth.

UNIT VII: KINETICS OF MICROBIAL GROWTH AND PRODUCT FORMATION

Phases of cell growth in batch cultures, Simple unstructured kinetic models for microbial growth, Monod model, Growth of filamentous organisms. Growth associated (primary) and non - growth associated (secondary) product formation Kinetics. Leudeking-Piret models, substrate and product inhibition on cell growth and product formation. Introduction to Structured Models for growth and product formation.

UNIT VIII: ENZYME PROCESSES

Production of enzymes in submerged and solid-state processes, extraction and purification of enzymes, methods of characterization, specific activity and activity definitions.

All relevant units will have basic numerical problems.

TEXT BOOKS

1. M.L.Shuler and F. kargi Bioprocess engineering, Prentice Hall of India 1992.
2. P.M. Doran, Biochemical process principles, Academic Press, 1995.
3. Harvey W. Blanch, Douglas S. Clark Biochemical Engineering, Marcecel, Dekker, 2007.
4. Bailey Ollis, Biochemical Engineering fundamentals, 2^nd edition, McGraw-Hill, 1986.

D.G.Rao, Introduction to Biochemical Engineering, McGraw-Hill,2005