INSTRUMENTAL METHODS OF ANALYSIS syllabus, JNTU (2007-2008)

UNIT I: INTRODUCTION

Types of Analytical Methods – Instruments for Analysis – Uncertainties in Instrumental measurements – Sensitivity and detection limit for instruments.

UNIT II: MICROSCOPY

Bright field, Dark field, Fluorescent, Phase contrast, confocal microscopy, SEM & TEM Microscopy, Flow Cytometry.

UNIT III: CENTRIFUGATION

General Principals, Ultra Centrifugation, velocity Sedimentation & measurements, Equilibrium Ultracentrifugation – Density Gradient centrifugation

UNIT IV : SPECTROSCOPY - I

General principles – Radiation, energy and atomic structure- types of spectra and their biochemical usefulness – basic laws of light absorption. Electromagnetic radiation & Spectrum, Beer – Lambert’s Law and apparent deviations; UV - VIS Spectrophotometer,Spectrofluorimetry, Atomic absorption & Atomic emission spectroscopy, Cirular Dichroism (CD)- principles, instrumentation and applications.

UNIT V: SPECTROSCOPY-II

Infra Red Spectroscopy. Mass spectroscopy-Introduction, analysis, applications in

biology ESR principles - instrumentation-applications

UNIT VI: ONLINE MONITORING AND CONTROL DEVICES

pH, temperature, dissolved oxygen, agitation, sensors and their operation.

UNIT VI: X RAY DIFFRACTION AND CRYSTALLOGRAPHY

Principle, Mode of Operation and Applications

UNIT VII: SEPARATION EQUIPMENTS – PRINCIPLES AND OPERATION:
HPLC, Gas chromatography, Ion – exchange Chromatography, Gel – filtration Chromatography, Affinity Chromatography, Membrane separations, Ultrafiltration , Reverse Osmosis

UNIT VIII: NMR

High resolution NMR –Chemical shift-Spin-spin coupling Frequency lock- double resonance-applications of proton NMR-quantitative analysis-qualitative analysis, application of NMR in biology and study of macromolecules

TEXT BOOKS:

1. A Biologist Guide to principles and techniques of practical Biochemistry. By

Keith Wilson, Kenneth H. Goulding 3rd ed. ELBS Series.

2. Skoog & West, Fundamentals of Analytical Chemistry, 1982

REFERENCES:

1. Vogel, Text Book of Quantitative Inorganic Analysis, 1990

2. Ewing, Instrumental Methods of Analysis, 1992

3. Hobert H Willard D. L. Merritt & J. R. J. A. Dean, Instrumental Methods of

Analysis, CBS Publishers & Distributors, 1992

4. F. Settle. Hand book of Instrumental techniques for Analytical Chemistry, Prentice Hall, 1997.