MOLECULAR BIOLOGY syllabus, JNTU (2007-2008)

UNIT I: STRUCTURE OF DNA

Detailed structure of DNA, variation from Watson & Crick model, Z - DNA, A & B DNA, Denaturation & melting curves.

UNIT-II: DNA REPLICATION - I

Models of DNA replication: semi conservative Mechanism of DNA replication in E.coli (bi- directional). Mitochondrial (D-loop), Viral DNA (Rolling circle), Single stranded- DNA phages (M13, Ø174),

UNIT – III : DNA REPLICATION- II

Eukaryotic telomeres and its replication Inhibitors of DNA Replication. Enzymes involved in replication, step by step process.

UNIT IV: RNA STRUCTURE AND BIOSYNTHESIS

m-RNA, r-RNA, t-RNA structures, Transcription apparatus, RNA polymerases and proteins involved in transcription (initiation, elongation and termination steps)

UNIT V : POST TRANSCRIPTIONAL PROCESSING

Post transcriptional processing of RNA ‘s t-RNA, r-RNA, m- RNA splicing. Inhibitors of transcription.

UNIT VI: PROTEIN BIOSYNTHESIS

The genetic code and Wobble Hypothesis, Codon usage, Protein synthesis In Prokaryotes.

UNIT VII : PROTEIN SYNTHESIS IN EUKARYOTES

Eukaryotic Protein synthesis, differences between prokaryotic and eukaryotic protein synthesis, Post translational modifications. Inhibitors of protein synthesis .

UNIT VIII : MUTAGENESIS

Mutations, spontaneous, induced, lethal, mutagens their types and actions, classification of mutations and their applications. Site - directed mutagenesis and reverse genetics. DNA damage and repair mechanisms. Mutagenicity testing using microbial systems, Ames TEST.

TEXT BOOKS

1. Molecular Biology, David Friefelder, Jones and Bartllett Publishing Home,1987.

2ShortProtocolsinMolecularBiology,2^ndeditionT..M.Ausubel,Brent,R.E.Kingston,D.D.Moire,J.G.Seidman,J.A.Smith, K.Struhl Green Publication Associates and John Wiley and sons 1991

REFERENCES:

1. Lodish, H., Berk A., Zipursky, S.L. Matsudaria, P. Baltimore, D. and Darnell, J.2003. Molecular Cell f Biology, Media connected, W.H. Freeman and Company.

2. Bio Chemistry and Molecular biology by William H. Ellioff. Oxford Publications

3. Cell and Molecular biology by Philip Sheeler Donald E.Bianchi Wiley Publishers.

4. Cell and Molecular Biology 1996. De Robertis E.D.Pi and De Robertis E.M.F.

B I Waverly Pvt. Ltd., New Delhi.