METABOLIC ENGINEERING Question Papers (Regular, 2005)

SET :1

1. Write short notes on :

a) Passive diffusion

b) Facultative diffusion

2. Describe in detail the primary screening involved in strain selection with example.

3. Describe

a) Differential regulation

b) Gene dosage

4. Explain mixed or sequential bioconversions with suitable examples.

5. Describe induction/repression phenomena in a E.coli with examples.

6. Describe enzyme inhibition and factors involved in it.

7. How does mutation effect the enzyme production? List out the factors involved in the optimization of mutants for high yield production?

8. Explain the fermentation parameters involved in production of wine from yeast.

SET : 2

1. Write short notes on :

a) Feed back repression

b) CAMP

2. Describe in detail the secondary screening involved in strain selection with example.

3. Detail the regulation of enzyme synthesis at fermentor level.

4. Define bioconversion and describe in detail the conversion of insoluble substances by sequential bioconversion.

5. Describe induction and repression phenomena in yeast, citing alchohol production as example.

6. Describe in detail the various modes of diffusion?

7. What is enzyme inhibition and detail the various modes of enzyme inhibition.

8. Distinguish and differentiate concerted feed back regulation and cumulative feed back regulation with examples.

SET : 3

1. Write short notes on :

a) Co-metabolism during bioconversion

b) Feed back regulation

2. List out and describe the parameters involved in scale up of fermentation (large scale) from pilot scale.

3. What is bioconversion and what are the advantages of molecules generated by bioconversion to industry.

4. Explain gene regulation by Jacob and Monad model citing lac operon as example.

5. Explain catabolic regulation with tryptophan operon as example.

6. Detail how gene dosage is evaluated and how does gene dosage effect fermentation process.

7. List out the biotechnological applications of enzymes (e.g. protease, amylase etc) produced by fermentation.

8. Distinguish and differentiate concerted and cumulative feed back regulation with examples.

SET :4

1. Write short notes on :

a) Strain selection

b) Isozymes

2. How can metabolic pathways be genetically controlled with examples.

3. What is bioconversion and describe the factors involved in bioconversion?

4. Describe

a) Concerted feed back regulation

b) Amino acid regulation

5. Describe the factors contributing to catalytic efficiency of an enzyme.

6. Define mutation and various modes of generating mutations in improving industrial biotechnology of an organism.

7. Compare and contrast direct and indirect fermentations citing amino acid synthesis as example?

8. Describe :

a) Precursor effects in biosynthesis of secondary metabolites.