METABOLIC ENGINEERING Question Papers (Supple, 2006)

SET : 1

1. What is bio-conversion? Discuss the advantages of bioconversion.

2. What is permeability? Discuss the alteration of permeability.

3. Discuss the biosynthesis of secondary metabolites.

4. What is growth cycle? How do you recognize growth cycle peaks?

5. Explain bio-conversion of insoluble substances.

Write about cAMP deficiency

6. Write short notes on the following:

a) Jacob Monod model

b) Passive diffusion

c) Resistant mutants

d) Catbolic repression

7. Describe the amino acid regulation of RNA synthesis.

8. Write short notes on :

a) Mutation

b) Cometabolism

c) Gene dosage

d) Feed back regulation

SET : 2

1. Define bio-conversion and what are the factors important to bio-conversions?

2. What is catabolic repression? Discuss mutants resistant to repression.

3. Describe the catabolite regulation in secondary metabolism?

4. Discuss the synthesis of primary metabolites.

5. Wtite about :

a) Differential regulation by isozymes.

b) Explain feed back regulation.

6. Write short notes on the following :

a) Precursor effect

b) Gene dosage

c) Co-metabolism

d) Permeability

7. Describe the metabolic regulation in branched pathways.

8. Write short notes on the following :

a) Resistant mutants

b) Diffusion

c) Mutation

d) Enzyme induction

SET : 3

1. Discuss the regulation of enzyme synthesis.

2. What is fermentation? What steps do you suggest for improving fermentation.

3. Discuss the biosynthesis of secondary metabolites.

4. What is permeability? Discuss the alteration of permeability.

5. Write about the following :

a) Feed back repression

b) Bioconversion of insoluble substances

6. Write short notes on the following :

a) Jacob Monod model

b) Bio-conversion

c) Gene dosage

d) Precursor effect

7. Describe the amino acid regulation of RNA synthesis.

8. Write short notes on the following :

a) Co-metabolism

b) Mutation

c) Diffusion

d) Strain selection.

SET : 4

1. Discuss the avoidance of product inhibition.

2. Discuss the role of strain in improving fermentation with some examples.

3. Explain the procedures of secondary metabolites.

4. What is permeability? Discuss the alteration of permeability.

5. Write about differential regulation by isozymes.

Define diffusion? Explain passive diffusion and facilitated diffusion.

6. Write short notes on the :

a) Jacob Monod model

b) Precursor effect

c) Catabolic repression

d) Gene dosage

7. Describe amino acid regulation of RNA synthesis.

8. Write short notes on the following :

a) Feed back regulation

b) Mutation

c) Bio-conversions

d) cAMP deficiency