METABOLIC ENGINEERING Question Papers (Regular, 2006)

SET : 1

1. Describe the primary screening involved in strain selection with example.

2. Write notes on :

a) Co-metabolism during bio-conversion

b) Concerted feed back regulation

3. Define bioconversion and list out in detail advantages of bio conversion in pharmaceutical industry with suitable examples

4. What is enzyme inhibition and detail various modes of enzyme inhibition.

5. Compare and contrast direct and indirect fermentations citing amino acid synthesis as example?

6. List out the biotechnological applications of enzymes (e.g. protease, amylase etc) produced by fermentation.

7. Detail on (with example)

a) Active transport

b) Group transport

8. List out organisms involved in production of secondary metabolites with detail on one secondary metabolite as example.

SET : 2

1. How can metabolic pathway be genetically controlled? Explain with two examples.

2. Detail

a) Passive diffusion

b) Isozymes

3. Explain gene regulation by Jacob and Monad model citing lac operon as example.

4. Detail how gene dosage is evaluated and how does gene dosage effect fermentation process.

5. Describe in detail the various modes of diffusion?

6. Explain fermentation parameter involved in production of wine from yeast.

7. How does mutation effect the enzyme production? List out the factors involved in the optimization of mutants for high yield production?

8. Distinguish and differentiate concerted feed back regulation and cumulative feed back regulation with examples.

SET : 3

1. List out and describe the parameters involved in scale up of fermentation (large scale) from pilot scale.

2. Describe

a) Gene dosage

b) Aminoacid regulation of RNA synthesis

3. Explain mixed OG sequential bioconversion with suitable example.

4. Describe catabolic repression with tryptophan operon as example.

5. Define mutation and various modes of generating mutations in improving industrial biotechnology of an organism.

6. Describe induction and repression phenomena with E.coli as an example.

7. How can permeability of an organism be altered in focus with primary metabolism.

8. Detail on

a) Glucose effect

b) Active transport

SET : 4

1. Describe in detail the secondary screening involved in strain selection with example.

2. Detail

a) CAMP

b) Feed back repression

3. Define bioconversion and describe factors involved in bioconversion with example(s).

4. What is antibiotic fermentation and explain its methodology in penicillin production.

5. List out and detail factors contributing to catalytic efficiency of enzymes.

6. How do mutations effect the enzyme production. List out aspects involved in optimization of mutants for high yield.

7. Describe in detail conversion of insoluble substance by mixed sequential bioconversion.

8. Detail

a) Fed batch fermentation

Continuous fermentation.