PLANT BIOTECHNOLOGY Question Papers (Regular, 2007)

SET :1

1. Write short notes on the following: [4 × 4]
(a) Clonal propagation
(b) Cell suspension cultures
(c) Synthetic seeds
(d) Organogenesis.

2. Discuss about a crop (e.g. Rice) plant where anther culture has been extensively used for practical applications. [16]

3. Discuss the strategy for the scaling up of a bioprocess for the production of secondary metabolites. [16]

4. Write in detail about the particle bombardment method of plant transformation. [16]

5. Discuss about the method for developing transgenic plants resistant to fungi. [16]

6. Describe how plants can be genetically engineered for submergence tolerance. [16]

7. Why do you think plants can be a suitable alternative to other living organisms for the production of biologically active compounds. [16]

8. Write short notes on the following: [4 × 4]
(a) Pharmaceuticals
(b) Fragrances
(c) Aroma
(d) Food additives.


SET :2

1. What do you understand by somatic hybridization what are its applications. [16]

2. Discuss a detailed protocol for isolated microspore culture. [16]

3. Do you think plant tissue culture can act as an alternative for the production of secondary metabolites. Explain. [16]

4. Give an account of gene transfer methods in plants for the production of transgenic plants. [16]

5. Discuss about the method for developing transgenic plants resistant to fungi. [16]

6. Describe how plants can be genetically engineered for submergence tolerance. [16]

7. Give a detailed account of production of phytase in transgenic plant and its use as a novel feed additive. [16]

8. Give an account of the genetic engineering of plants for production of vitamin E. [16]


SET :3

1. What are cybrids. How cybrids can be developed by protoplast fusion. [16]

2. Discuss the process of cryopreservation in detail and write about its implications in the germplasm conservation of plants. [16]

3. Discuss different in vitro culture systems for the production of secondary metabolites. [16]

4. Write a detailed note on chemical method of gene transfer emphasizing on PEG mediated DNA uptake. [16]

5. Discuss about the method for developing transgenic plants resistant to fungi. [16]

6. Give an strategic plan by which the limitation of abiotic stress in crop plants can be addressed by transgenic approach. [16]

7. What is the biotechnological application of hirudin. Describe the production of recombinant hirudin from plant seeds. [16]

8. Write short notes on the following: [4 × 4]
(a) Pharmaceuticals
(b) Fragrances
(c) Aroma
(d) Food additives.


SET :4

1. What is clonal propagation; discuss different stages of clonal propagation. [16]

2. Suggest ways of enhancing naturally occurring genetic variations with desirable characteristics using tissue culture. [16]

3. Discuss different in vitro culture systems for the production of secondary metabolites. [16]

4. Give a detailed note on plant transformation vectors. [16]

5. Discuss about the method for developing transgenic plants resistant to fungi. [16]

6. What are stress inducible transcription factors, give a detailed account of dehydration response element (DRE) and its role in the expression of gene to abiotic stress.[16]

7. Why do you think plants can be a suitable alternative to other living organisms for the production of biologically active compounds. [16]

8. Give an account of the genetic engineering of plants for production of vitamin E. [16]