ENZYME ENGINEERING Question Papers (2006,Reg)

SET :1

1. Write about the applications of enzymes in food industries.
2. Describe the procedures of enzyme isolation from natural sources.
3. Discuss different types of enzyme specificity with suitable examples.
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed reaction.
5. Discuss allosteric enzymes in terms of co-operativity.
6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization.
7. Discuss the design of enzyme electrodes.

SET :2

1. Discuss enzyme classification in terms of 4-digit classification number?
2. Describe the procedures of enzyme isolation from natural resources.
3. Discuss why the lock and key model could lead to an inefficient enzyme mechanism and induced fit model to an efficient enzyme mechanism.
4. Derive the general rate equation of Alberty for multi-substrate enzyme catalyzed reaction.
5. Discuss and compare the non-competitive and uncompetitive inhibition based on their kinetics.
6. Discuss in detail about the cross linking method for enzyme immobilization.
7. Give an account of different types of immobilized enzyme reactors.
8. How enzyme electrodes are useful in health care industry as biosensor.

SET :3

1. Discuss the enzyme classification in terms of 4-digit classification number?
2. Discuss the methods employed for isolating enzymes soluble in cytoplasm and subcellular organelles from animal, plant and microbial sources.
3. Enumerate Lock & Key model and Koshland's Induced fit model.
4. Derive Michaelis-Menten equation. State the importance of MM constant.
5. How Koshland-/nemethy-Filmer (KNF) model account for allosteric regulation.
6. Discuss about the medical application of immobilized enzymes.
7. Give an account of different types of immobilized reactors.
8. Enumerate the application of enzymes in analysis

SET :4

1. Enumerate the diagnostic importance of enzymes.
2. Describe the procedures of enzyme isolation from natural resources.
3. Discuss different types of enzyme specificity with suitable examples.
4. Discuss the kinetics for reversible reactions.
5. How Koshland-/nemethy-Filmer (KNF) model account for allosteric regulation.
6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization.
7. Discuss about the dynamic models of bioreactors.
8. Enumerate the application of enzymes in analysis