ENZYME ENGINEERING Question Papers (2006,Supple)

SET :1

1. State the importance of enzymes in medical diagnosis.
2. Describe the procedures of enzyme isolation from natural resources .
3. Give an account of various factors that affect enzyme catalysed reaction.
4. Write the significance of MM equation.
5. Explain various types of enzyme inhibition with relevant examples.
6. Discuss the important criteria and prerequisite for selecting support for enzyme immobilization.
7. Discuss about the dynamic models of bioreactors.
8. Discuss the design of enzyme electrodes.

SET :2

1. What are enzymes? Justify its role as biocatalysts.
2. Describe the procedures of enzyme isolation from natural resources.
3. Give an account of various factors the affect enzyme catalysed reaction.
4. Derive Michaelis-Menten equation. State the importance of MM constant.
5. How Koshland-/nemethy-Filmer (KNF) model account for allosteric regulation.
6. Discuss about the interactions and carriers used for enzyme immobilization by adsorption.
7. Discuss the design and application of Packed bed reactors.
8. What are the different types of transducers used in biosensors?

SET :3

1. Discuss the importance of enzymes in pharmaceutical industry?
2. How are enzymes characterized? what are the importances of enzyme characterization?
3. Discuss different types of enzyme specificity with suitable examples.
4. Derive Michaelis-Menten equation. State the importance of MM constant.
5. How MWC model account for cooperativity and explain allosteric regulation.
6. Discuss the important criteria and prerequisite for selecting suppourt for enzyme immobilization.
7. Give an account of different types of immobilized enzyme reactors.
8. Enumerate the application of enzymes in analysis.

SET :4

1. State the importance of enzymes in medical diagnosis.
2. How enzymes are preliminarily purified from a crude enzyme extract?
3. Discuss why the lock and key model could lead to an inefficient enzyme mechanism and induced fit model to an efficient enzyme mechanism.
4. Discuss about the Eadie-Hofstee and Hanes Plot and state their edge over the LB plot.
5. What are allosteric enzymes? Discuss their role in the regulation of a metabolic pathway.
6. Discuss the important criteria and prerequisite for selecting suppourt for enzyme immobilization.
7. Give an account of different types of immobilized enzyme reactors.
8. How enzyme electrodes are useful in health care industry as biosensor.