ENZYME ENGINEERING Question Papers (2008,Reg)

SET : 2

1. Write about the one-dimensional and two-dimensional electrophoresis in protein purification?
2. Explain in detail about the isolation of enzymes from animal cells.
3. What is an active site of an enzyme? How the active site involves in enzyme substrate complex formation. Explain in detail about the determination of active site concentration.
4. Show pictorially the Dixon Plot for competitive inhibitors and explain in detail.
5. a) What are the factors that determine the adsorption of enzyme to the supports in the immobilization of enzymes by adsorption method. b) Describe the covalent method of enzyme immobilization on trialcoxysilanes.
6. Discuss the external diffusional effects on the immobilized enzyme kinetics on non-porus supports.
7. Discuss the use of enzyme bound membrane reactors in the reactions involving organic solvents.
8. Write an assay on the use of enzymes as biosensors