ENZYME ENGINEERING Question Papers (2006,Supple)

SET : 1

1. Explain the enzymw classification with examples for each class.

Explain the terms apoenzyme, holoenzyme, coenzyme and prosthetic group.

2. Explain the different methods available for enzyme purification.

How do you characterize an enzyme?

3. Discuss the application of enzymes in food, pharma and medical fields.

How are enzymatic assays developed? Discuss with an example.

4. What are the different types of inhibition.

Discuss briefly the kinetics of single substrate reactions and multiple substrate reactions.

5. Explain the following :

a) Allosteric regulation

b) Deactivation enzymes

c) Energitics of enzyme substrate complex formation.

d) Factors affecting enzyme activity

6. What are the different techniques used for enzyme immobilization? Comment on the advantages and disadvantages of each of these techniques.

7. Write short notes on :

a) Applications of immobilized enzymes

b) Uses of enzymes in different areas.

8. Comment on any two of the following :

a) Mass transfer effects in immobilized enzymes.

b) Biosensors

c) Applications of enzyme electrodes.

SET : 2

1. Discuss the six main classes of enzymes.

Comment on the applications of enzymes in pharmaceutical insustries.

2. Define the terms :

a) Turnover number

b) Michaelis menten constant.

Discuss the different types of enzyme inhibition.

3. What are the different immobilization techniques that can be used for immobilization?

Comment on the different applications of immobilized enzymes.

4. Write notes on :

a) Fluidized – bed reactors

b) Packed bed reactors.

5. What are biosensors? Discuss their applications in different areas.

6. Derive the michaelis menten equation.

Discuss allosteric enzymes and the kinetics of allosteric enzymes.

7. Write a note on the development of enzyme assays.

Discuss the different methods involved in purification of enzymes.

8. Write notes on :

a) Mechanism of enzyme action.

b) Applications of enzymes in analysis.

SET :3

1. What are enzymes? How are they classified? Explain.

How are enzymes extracted from various sources? What precautions are required to be taken while isolating the enzymes?

2. Explain what you understand by the following :

a) Turnover number

b) Michelis menten constant

c) Prosthetic group

d) Allosteric enzymes

3. Discuss the uses of enzymes in different areas.

What do you understand by the terms competitive and noncompetitive inhibition? Explain.

4. Write short notes :

a) End product inhibition

b) Significance of end product inhibition in biological systems.

c) Applications of immobilized enzymes.

d) Activation energy.

5. Derive the michaelis menten equation.

How do the kinetics differ in the case of reactions catalyzed by allosteric enzymes.

6. Discuss the various physical and chemical techniques used for enzyme immobilization.

7. Write notes on :

a) Kinetics of immobilized enzyme reactors.

b) Immobilized enzyme reactors.

8. Discuss the principle behind design of enzyme electrodes and their applications as biosensors in different areas.

SET :4

1. Comment on the applications of enzymes in different areas.

How can you obtain crude enzyme extracts from different sources? Discuss with examples?

2. Derive the Michaelis – Mente equation.

Discuss different types of enzyme inhibition with kinetic models.

3. Explain the following :

a) Turnover number

b) Deactivation of enzymes.

c) Allosteric enzymes.

d) Allosteric regulation.

4. Discuss the physical techniques available for enzyme immobilization.

Comment on the advantages and disadvantages of enzyme immobilization.

5. Discuss the design of immobilized enzyme reactors.

Discuss the mass transfer in immobilized enzyme reactors.

6. Discuss the methods involved in enzyme purification.

7. Write short notes :

a) Development of enzyme assays.

b) Characterization of enzymes

8. Design of enzyme biosensors.

Application of biosensors in different areas.