MOLECULAR BIOLOGY Question Papers (2008, Aug/Sep, Supple)

SET: 1

1. Represent a melting curve of a double helix DNA and explain it in detail?

2. Write a note on the famous Messelson and Stahl experiment on proving semi conservative mode of DNA replication?

3. what is the mechanism and importance of 'proof reading' function of DNA polymerase in DNA replication

4. With respect to tRNA structure, explain:

• Stems and loops
• Anticodon arm.

5. Which are the different types of rRNA associated with eukaryotic ribosome? How are these synthesized?

6. How are Wooble hypothesis ans the genetic code correlated with each other?

7. How is the process of eukaryotic process of translation different from prokaryotic process?

8. With context to mutations, what is meant by:

• Base substitutions
• Insertions and deletions

SET: 2

1. Which from of DNA exists as the natural form present in living cells? Explain in detail.

2. What is meant by 'rolling circle' form of DNA replication? Give an example.

3. What is meant by 'end replication problem' in DNA synthesis? Discuss the role of telomase in it.

4. How are eukaryotic and prokaryotic RNA Polymerases different from each other?

5. What is the relationship of intron and exon sequences with respect to the final mRNA formation in eukaryotic post translational processing?

6. Explain how the genetic information present in the mRNA is decoded into amino acid sequence during the process of translation?

7. What are post translational modifications? Give some examples.

8. Briefly explalin the different DNA repair mechanisms existing in a cell.

SET: 3

1. What is the primary structure of DNA? Explain in detail.

2. Write a note on the famous Messelson and Stahl experiment on proving semi conservative mode of DNA replication?

3. Discuss the various enzymes involved in carrying out the process of the DNA replication.

4. Discuss about the different types of RNA and the RNA Polymerases, which are involved in synthesising them.

5. Write a note on how tRNA is produced by processing larger pre-tRNA transcript.

6. How are Wooble hypothesis ans the genetic code correlated with each other?

7. Explain how conjugation is a process through which genetic recombination is possible in bacteria?

8. With reference to acting as mutagens, explain:

• UV irradiation
• Ionizing radiation

SET: 4

1. What are the main functions of DNA in a living cell?

2. How does DNA replication occur in Mitochondria DNA?

3. what are telomers in DNA? How do they replicate?

4. Write notes on:

• Prokaryotic RNA Polymerase
• Eukaryotic RNA Polymerase

5. Explain the sequence by which introns are removed from a eukaryotic pre-mRNA.

6. Elaborate the statement "Genetic code is a triplet, redundant, non-overlapping and comma free code".

7. Write short notes on :

• tRNA as an adaptor molecule
• Difference between prokaryotic and eukaryotic ribosomes

8. With reference to acting as mutagens, explain:

• UV irradiation
• Ionizing radiation