PHARMACEUTICAL ANALYSIS - II (PRACTCAL)

1. Separation of plant materials by column chromatography
2. Separation and identification of flavanoids/sulphonamides by paper chromatography
3. Separation and identification of sulphonamides by paper chromatography
4. Separation and identification of amino acids by TLC methods
5. Separation and identification of barbiturates by TLC methods
6. Determination of λ max, (KMnO4 and methylene blue solutions)
7. Demonstration experiments in HPLC and GLC
8. Assay of sulphadiazine tablets by visible spectrophotometry
9. Assay of sulphadiazine tablets by UV spectrophotometry
10. Demonstration experiments in IR spectrometry including interpretation of given spectra.
11. Fluorimetric estimation of quinine sulphate in formulations
12. Fluorimetric estimation of riboflavin in formulations
13. Flame photometric estimation of sodium and potassium ions
14. Potentiometric analysis a) Determination of pH of two solutions b) Titration of strong acid against strong base c) Titration of strong base against weak acid d) Simultaneous determination of strong acid and weak acid in a mixture e) Potentiometric assay of any two formulations from I.P.
15. Conductometric titration of NaOH with HCl
16. Polarographic estimation of drug official in I.P.
17. Determination of concentration of sugar solution by polarimetry
18. Determination of critical micellar concentration (butyric acid in water using abbe refractometer)
19. Demonstration experiments in detection of polymorphism and pseudo polymorphism in pharmaceuticals by DTA and DSC
20. Assay of an ointment and cream official in I.P.
21. Complete testing and assay of any two drugs as per I.P. monograph.

Reference books:
1. A text book of pharmaceutical analysis by K.A.Connors (John Wiley)