PHARMACEUTICAL BIOTECHNOLOGY Question Papers 4 A.U

Answer question no.1(compulsory) and any four of the remaining. All questions carry equal marks

1. With the help of a neat diagram explain the different parts of a fermenter and mention this functions. Add notes on the different types of fermenters used.

2. Describe in detail the fermentative production, recovery and applications of citric acid.

3. Discuss about different methods for immobilization of enzymes. Write about the advantages and disadvantages of immobilization.

4. Explain the production of Interferons by r-DNA technology.

5. Define enzymes and discuss this properties. Explain general methods of their preparation and applications in pharmacy, therapeutics and clinical analysis.

6. Write notes on:

• Mutagens and their mechanism of action.
• Transformation with recombinant plasmid DNA
• DNA Probes for diagnosis of genetic diseases
• Southern blotting and Northern blotting.

7. Write short notes on:

• Explain the process of protoplast fusion and its applications.
• Methods of cutting and joining of DNA molecules.

8. What is plasmid vector. Write about the properties of plasmids and desirable characteristics of a cloning vector. Discuss about natural and artificail plasmids.