DOWNSTREAM PROCESSING syllabus, JNTU (2007-2008)

UNIT I: ROLE OF DOWNSTREAM PROCESSING IN BIOTECHNOLOGY

Role and importance of downstream processing in biotechnological processes. Problems and requirements of bioproduct purification. Economics of downstream processing in Biotechnology, cost-cutting strategies, characteristics of biological mixtures, process design criteria for various classes of bioproducts (high volume, low value products and low volume, high Value products)

UNIT-II:

Physico-chemical basis of bio-separation processes. Recent development in product

Isolation (for ex. one step purification, reverse Micro cellular extraction on line membrane separation).

UNITIII: PRIMARY SEPARATION AND RECOVER PROCESS

Cell disruption methods for intracellular products, removal of insoluble, biomass (and particulate debris) separation techniques, flocculation and sedimentation, centrifugation and filtration methods.

UNIT IV: MEMBRANE SEPARATIONS

Membrane-based separations (micro and ultrafiltration), theory, design and configuration of membrane separation equipment applications,

UNIT V: ENRICHMENT OPERATIONS

Precipitation methods (with salts, organic solvents, and polymers, extractive separations, aqueous two-phase extraction, supercritical extraction), in situ product removal, integrated bioprocessing.

UNIT VI: ELECTROPHORESIS

Electrophoresis of proteins and nucleic acids, 1D-2D Gels, Types of Electrophoretic techniques (Capillary and Pulse field).

UNIT VII: PRODUCT RESOLUTION / FRACTIONATION

Chromatographic techniques- Paper, TLC, Adsorption, Ion exchange, Gel filtration, affinity chromatographic separation processes, GC, HPLC, FPLC, Chromatofocusing electrophoretic separations.

UNIT VIII: NEW AND EMERGING TECHNOLOGIES

Dialysis, Crystallization Pervaporation, super liquid extraction foam based separation case study with examples for processing of Two Industrial Products (Citric acid / Penicillin and Low volume high value product like recombinant proteins).

TEXT BOOKS:

1. Wankat PC. Rate controlled separations, Elsevier, 1990.

2. Belter PA and Cussler E. Bioseparations, Wiley 1985.

REFERENCES:

1. Product Recovery in Bioprocess Technology, BIOTOL.’ Series, VCH, 1990.

2. Asenjo J.M. Separation processes in Biotechnology, 1993, Marcel Dekkere Inc

3. M.R.Ladisch, Bioseparation engineering: Principles, Practice and Economics, Wiley Interscience 2001