SET: 1
1. Write how attenuation controls expression of Trp operon.
2. What is signal transduction? How does it help in regulating gene expression?
3. Write short notes on any two:
a) Relaxed plasmid
b) PUC 8
c) α- Complementation
4. Write C- DNA method of gene cloning.
5. Write short notes on any two:
a) Restriction enzymes
b) Gel electrophoresis
c) Immunoflorescence
6. Repeated
7. Repeated
8. Discuss about the viral methods used in doing in vivo gene therapy.
SET: 2
1. Explain the role of DNA binding proteins in genetic engineering.
2. What is signal transduction? How does it help in regulating gene expression?
3. Write short notes on any two:
d) Relaxed plasmid
e) PUC 8
f) α- Complementation
4. write short notes on any two:
a) SV 40 virus
b) Genomic library
c) C- DNA
5. Write about the vectors used in a gene library construction.
6. What is meant by PCR? Describe its methodology.
7. Discuss the application of gene chips in various fields of biotechnology.
8. Give detailed information about any two protein products, which are produced through r DNA technology.
SET: 3
1. Write notes on any two:
a) Terminator loops
b) Leader peptide
c) Trp. Repressor
2. Explain the role of peptide hormones in eukaryotic gene regulation.
3. What are the characters of ideal plasmid? Explain with suitable example.
4. Write detailed account on restriction mapping and its importance.
5. Explain the expression of cloned genes in yeast using suitable examples.
6. How is PCR different from the traditional cloning?
7. What is repetitive DNA? Discuss its significance in a genome.
8. Differentiate between In Vivo & Ex Vivo gene therapy.
SET: 4
1. Repeated
2. Write any two:
a) Tissue specific enhancers
b) Phosphorylated proteins
c) TATA box
3. Repeated
4. Write any two:
a) Restriction digestion
b) Homo-polymer tailing
c) Ligases
5. Repeated
6. Compare and contrast between PCR & RT-PCR
8. Write in detail about Agro-bacterium based plant vectors.
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