SYLLABUS 4 JNTU BME - APPLIED CHEMISTRY AND BIOCHEMISTRY

APPLIED CHEMISTRY AND BIOCHEMISTRY

UNIT-I
Electro Chemistry: Conductance, Specific Equivalent and Molecular Conductance -Cell constant -Measurement of Equivalent Conductance –Galvanic Cell
Standard Electrode Potentials -Calculation of E. M. F of a cell –Determination of pH by E. M. F methods.

UNIT-II
Polymers: applications of Polyethylene, Teflon, PVC, Bakelite, Rubber -Natural Rubber.

UNIT-III
Lubricants and water Technology: Definition -Theory of Lubrication, properties of Lubrication. Nature of Impurities present in water, Hardness of water and its Determination, Different methods of Treatment of water- Lime soda, Zeolite, Ion exchange process- Analysis of water.

UNIT-IV
Biochemistry of Living Cell, Sub-cellular. Fractionation using the Differential Centrifugation Method. Functions of each Organelle, Transport of Substances across Biological Membrane Function. Redox potential, Oxidative phosphorylation.
UNIT-V
Chemical nature of Enzymes - study of the Properties of Enzymes and kinetics by Spectrophotometer. Diagnostic and therapeutic uses of Enzymes.

UNIT-VI
Carbohydrate, Protein & Lipid metabolism (Briefly), Nucleic Acid chemistry. Protein synthesis. Transcription and Translation, Replication, Polymerase Chain Reaction(PCR) Immunological Techniques or Immunoassay – Radio Immuno Assay (RIA), Enzyme- Linked Immunosorbent Assay (ELISA), Chemiluminiscence.

UNIT-VII
Blood Chemistry: Chemical Composition of Blood, Separation of Serum Proteins and lipoproteins by Electrophoresis and Ultracentrifugation Acid Base Balance and Biochemical Measurements of Acid-Base and Electrolyte status of the patients, Urine Analysis.

UNIT-VIII
General methods of biochemical analysis carried out in the estimation of blood constituents, such as glucose etc. Principles and different methods of chromatography – fluorometry, flame photometry, Automation and Biochemical Analysis. Applications of isotopes in biochemistry.

TEXT BOOKS
1. Robert Murray, Peter A. Mayes, Victor W. Rodwell, Daryl K. Granner, Harper’s Biochemistry, 26th Edition, McGraw-Hill Companies, February 2003.
2. Jain & Jain, Engineering Chemistry” Dhanpat Rai Publishing company,. New Delhi.

REFERENCE
1. Rao, N. Mallikarjuna, Medical Biochemistry, New Age International Pvt Ltd,2002.
2. Lalit M. Srivastava, Nibhriti Das & Subrata sinha, Essentials of practical Biochemistry, CBS Publishers, Ist edition, 2002