SET :1
1. What are the various products of r DNA and their economic assessment of various proteins? [16]
2. Describe about primary factors affecting separation? [16]
3. Write in detail about physico-mechnaical methods of Cell disruption? [16]
4. Explain about Cell aggregation and Flocculation? [16]
5. Write about different modes of operation of filters? [16]
6. Explain the various factors that influence protein solubility? [16]
7. Write short notes on:
(a) Paper chromatography
(b) SDS-PAGE [8+8]
8. Explain about the process of Dialysis? [16]
SET :2
1. Describe about preliminary economic analysis or cost analysis performed in Bioprocess? [16]
2. Explain the role of Downstream processing and different market sectors in Biotechnology? [16]
3. Explain with a neat sketch the working of Homogenizer? [16]
4. Explain about Cell aggregation and Flocculation? [16]
5. Write short notes on:
(a) Reverse Osmosis
(b) Ultra filtration [8+8]
6. Describe the importance of process integration and whole broth treatment and in-situ recovery strategies for product recovery? [16]
7. Write short notes on:
(a) Paper chromatography
(b) SDS-PAGE [8+8]
8. Explain about the process of Dialysis? [16]
SET :3
1. Examine the continuing role which economics plays in Bioprocess research, development and commercialization? [16]
2. Explain the role of Downstream processing in Biotechnology? [16]
3. Write in detail about physico-mechnaical methods of Cell disruption? [16]
4. What are the various techniques employed in recovery of Bioproducts? Explain in brief about them? [16]
5. Write about different modes of operation of filters? [16]
6. Discuss about Colloidal stability of protein solutions? [16]
7. Write short notes on:
(a) Rocket Immuno electrophoresis
(b) Capillary electrophoresis [8+8]
8. Write short notes on:
(a) Secondary nucleation
(b) Heterogeneous nucleation [8+8]
SET :4
1. Examine the continuing role which economics plays in Bioprocess research, development and commercialization? [16]
2. Explain about the Filtration technique employed for several Bioproducts? [16]
3. Write short notes on:
(a) Homogenizer
(b) Bead mill [8+8]
4. What are the Factors influencing Sedimentation rates of particles in Batch gravity? [16]
5. How can RO and Ultra filtration complement each other? [16]
6. Describe about different techniques employed in Precipitation? [16]
7. Write short notes on:
(a) Paper chromatography
(b) SDS-PAGE [8+8]
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