DOWN STREAM PROCESSING QUESTION PAPERS (SUPPLE, 2008)

SET : 1

1. Describe about economic assessment of various proteins via rDNA?

2. Explain the role of Downstream processing and differentiate market sectors in Biotechnology?

3. How can you classify cell disruption methods? Explain in brief about them?

4. Write about the pressure leaf filters?

5. Explain about the Basic principles of membrane separation?

6. Discuss about colloidal stability of protein solutuions?

7. Write short notes on :

a) Paper chromatography

b) SDS – PAGE

8. Describe about the process of electrodialysis?

SET : 2

1. Explain the different stages in downstream processing and briefly describe about techniques employed in each stage?

2. Explain about the filtration technique employed for several Bioproducts?

3. Write in detail about the Non – Mechanical methods of cell disruption?

4. What are the various techniques employed in recovery of bioproducts? Explain in brief about them?

5. Write short notes on :

a) Reverse osmosis

b) Ultra filtration

6. Describe about different techniques employed in precipitation?

7. Describe the following :

a) Liquid adsorption chromatography

b) LLC

8. Explain about the process of Dialysis?

SET : 3

1. Examine the continuing role which economics play in bioprocess research, development and commercialization?

2. Explain about the filtration technique employed for several bioproducts?

3. Explain with a neat sketch the working of a homogenizer?

4. What are the various techniques employed in recovery of bioproducts? Explain in brief about them?

5. Explain & describe the following membrane separation process RO and Ultra filtration and clearly delineate between the different process?

6. Describe about different techniques employed in precipitation?

7. Derive the mass balance for fixed bed adsorption?

8. Explain about the process of Dialysis?

SET : 4

1. Explain in detail about bioprocess economics and evaluation of market?

2. Explain the principle of solvent extraction and its applications with an example?

3. Explain about working of a bead mill and on what factors does the optimal conditions in a bead mill depends upon?

4. Write short notes on :

a) Tubular centrifuge

b) Multi chamber centrifuge

5. Write short notes on :

a) Reverse osmosis

b) Ultra filtration

6. Describe about different techniques employed in precipitation?

7. Explain in brief about different methods for electrophoresis?

8. Out line the strategies for crystallization of proteins.