DOWN STREAM PROCESSING QUESTION PAPERS (REGULAR, 2006)

SET : 1

1. Examine the continuing role which economics play in bioprocess research, development and commercialization?

2. Describe about extraction using aqueous two phase systems?

3. Explain about working of a bead mill and on what factors does the optimal conditions in a bead mill depends upon?

4. Write short notes on :

a) Basket centrifuge

b) Solid bowl scroll centrifuge

5. What are the different types of membranes available for separations and explain about different membrane configurations?

6. Explain the concept of DLVO theory?

7. Explain about the basic concepts of Electrodialysis?

8. Explain about the process of Dialysis?

SET : 2

1. Describe about economic assessment of various proteins via rDNA?

2. Give generalized process recovery scheme for enzymes derived from animal, plant, surface or submerged fermentations?

3. How can you classify cell disruption methods? Explain in brief about them?

4. Write about the pressure leaf filters?

5. How can RO and Ultra filtration complement each other?

6. Describe about different techniques employed in precipitation?

7. Write short notes on :

a) Distribution coefficients

b) Modes of chromatography

8. Explain about the process of Dialysis?

SET : 3

1. Examine the continuing role which economics play in bioprocess research, development and commercialization?

2. Explain about the filtration technique employed for several Bioproducts?

3. What is meant by cell disruption & explain about the functioning of bead mill?

4. Explain about cell aggregation & Flocculation?

5. Write short notes on :

a) Liquid membranes

b) Ultra Filtration

6. Describe about different methods for extraction of bioproducts?

7. Derive the mass balance for fixed bed adsorption?

8. Explain & classify the types of crystallization used based on how supersaturation is achieved?

SET : 4

1. Describe about the preliminary economic analysis or cost analysis performed in bioprocess?

2. Explain about the filtration technique employed for several Bioproducts?

3. Write short notes on :

a) Enzymatic cell lysis

b) Chemical cell lysis

4. What is the difference between sedimentation and centrifugation and list the advantages of centrifugation?

5. Write about different modes of operation of filters?

6. Describe about different agents employed in precipitation ?

7. Explain about Affinity electrophoresis and immunoelectrophoresis?

8. Describe about the process of electrodialysis?