DOWNSTREAM PROCESSING Question Papers (REG, 2008)

SET: 1

1. Describe about economic assessment of various proteins via rDNA.

2. (a) What are the parameters used in characterization in fermentation broth? (b) List down all the important impurities and contaminants present in DSP and their removal techniques.

3. Write short notes on the following.
(a) enzymatic cell lysis
(b) chemical cell lysis
(c) concentration polarization and cross flow filtration.

4. Write short notes on :
(a) Liquid membranes
(b) Ultra filtration

5. (a) Write about the criteria used for selection of extraction equipment in antibiotic industry.
(b) A compound X is to be extracted from a clarified fermentation beer by using pure amyl acetate as solvent at pH 4.0. The distribution coefficient K of the system was found to be 30. The initial concentration of compound x in the feed is 400 ml/L. The flow rates of the feed and solvent streams are 500L/H and 30L/H respectively. How many ideal stages are required to recover 90% of X in the feed.

6. Write the mode of separation of compounds by capillary electrophoresis. Discuss the mode of operation by a schematic diagram.

7. write short notes on:
(a) Peak asymmetry
(b) Selectivity factor
(c) Stationary phase
(d) Gradient elution.
8. Discuss the process of crystallization. What are the different parameters considered before crystallising a compound?


SET: 2

1. What is the role of process engineer in Bioseparation process? Explain in detail with help of suitable examples.

2. What are the major steps involved in the product isolation and purification of citric acid manufacture?

3. (a) Discuss the theoretical principles of constant pressure filtration. (b) How is compressibility of a cake determined?

4. Explain in detail how do you classify the membrane separation methods according to particle size.

5. What is integrated bioprocessing. Draw a neat schematic diagram for a known compound of your interest. What are the likely problems encountered in the process and the necessary steps to overcome them to upkeep the process efficiency.

6. Write the procedure of SDS - PAGE. Discuss how to calculate the molecular weight of an unknown protein on the gel.

7. (a) Write the principle of Gel chromatography. (b) What is the retention volume (VR) if external solution (Vo) is 16ml and internal solution is 5ml and fraction of (Vi) acceptable to solute is 12 ml in a gel chromatography column?

8. (a) Write about the process of crystal formation and the geometrical changes associated with it. (b) Write the significance of crystallization in product recovery.


SET: 3

1. Discuss about the classical and modern biotechnology consideration of downstream processing in biotech industry.

2. Discuss the steps involved in the product isolation and purification of an antibiotic production.

3. Write notes on the operation of tubular bowl centrifuge and disc stack centrifuge.

4. (a) Discuss about the classification of membrane separation process. (b) What are the transport models for membrane process? Explain about them.

5. Explain the "in situ product removal as a tool for bioprocessing".

6. (a) Describe about different types of support media employed in electrophoresis. (b) How to prepare the sample of a nucleic acid to load on an agarose gel.

7. (a) Write about different types of stationary phases available for gas chromatography. (b) what is the percentage composition of the mixture of Ethane, propane, butane if in gas chromatography seperation the peak areas were 53.2, 14.5 and 31 cm.

8. Write the principle and process of crystallization. Discuss how it is different from precipitation.


SET : 4

1. Give generalized process recovery scheme for enzymes from plant resources with help of solid state fermentation.

2. Write in detail about the selecting of various unit operations in relation to their separation factor and the molecular size of the material to be recovered in DSP.

3. Explain the principle of centrifugal seperation. What is a Gyrotester? What are its uses?

4. (a) What are the advantages of membrane separation processes (b)What is Ultrafiltration? How is it useful in bioseperation?

5. What is in situ product removal in bioprocessing. What are the disadvantages of using ISPR and the necessary precautions in ISPR operation.

6. Short notes on: a) Capillary array Electrophpresis. b) Isoelectric focusing c) Capillary zone Electrophoresis d) Electro osmotic flow.

7. Write short notes on : a) Temperature programming in G.C a) Band seperation c) Electron Capture detector. d) Open tubular column.

8. What is crystallization and how it is common and different from lyophilization.